Cookies disclaimer

Continue Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device. Additional information can be found under the cookie section of this page.

Kidney toxicity testing and best practices    Back

CAAT Academy Course Type: Short courses (CPD) Start Date: To be announced
Duration: Full time - 2 Days Fees/total cost: EUR 590.00



  • Lecture/Regulatory aspects

         - From AOPs to IATAs-future regulations of chemicals with integrated in silico and in vitro methods:       Martin Paparella

  • Lecture/Kidney models

         - Primary human RPTECs: Eva-Anne Subileau

         - hTERT immortalized human renal proximal tubular epithelial cells (RPTEC/TERT1) and           urine derived kidney models: Matthias Wieser

         - High-throughput microfluidic platform for culture of 3D kidney tissue models:                                                Henriëtte Lanz

  • Lecture/Nephrotox

         - Integrated and omics and biokinetics for understanding mechanisms of chemical   induced                       nephrotoxicity: Paul Jennings

         - Raman microspectroscopy – new tools in toxicology: Regina Weinmüllner


Hands-on trainings (HOT)

  • 2D culture of renal cells: primary RPTECs, RPTEC/TERT1 cells, urine-derived kidney cells (thawing, passaging, seeding into plates, chemical treatment, TEER measurement): Paul Jennings, Eva-Anne Subileau, Matthias Wieser
  • 3D culture of renal cells: spheroid culture of primary RPTECs (Eva-Anne Subileau) and seeding of kidney tubules in an OrganoPlate® (Henriëtte Lanz)
  • Toxicity test: resazurin assay, analysis if lactate production; evaluation of cellular morphology, raman microspectroscopy, data analysis and interpretation (Paul Jennings, Regina Weinmüllner)

  Provided by: Medical University of Innsbruck, Environmental Agency Austria (Umweltbundesamt), Evercyte, Biopredic, Mimetas, BOKU-VIBT

Learning Outcomes

The 2 days training will give a comprehensive overview of the most relevant and robust 2D and 3D kidney cell culture models and in vitro tests applications for the assessment of kidney-associated cytotoxicity.

Course Provider Details

Course contact information

Dr Francois Busquet
CAAT Academy coordinator
University accredited:
Last update: 25/08/2016