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Kidney toxicity testing and best practices    Back

CAAT Academy Course Type: Short courses (CPD) Start Date: To be announced
Duration: Full time - 2 Days Fees/total cost: EUR 590.00

Description

Lectures

  • Lecture/Regulatory aspects

         - From AOPs to IATAs-future regulations of chemicals with integrated in silico and in vitro methods:       Martin Paparella

  • Lecture/Kidney models

         - Primary human RPTECs: Eva-Anne Subileau

         - hTERT immortalized human renal proximal tubular epithelial cells (RPTEC/TERT1) and           urine derived kidney models: Matthias Wieser

         - High-throughput microfluidic platform for culture of 3D kidney tissue models:                                                Henriëtte Lanz

  • Lecture/Nephrotox

         - Integrated and omics and biokinetics for understanding mechanisms of chemical   induced                       nephrotoxicity: Paul Jennings

         - Raman microspectroscopy – new tools in toxicology: Regina Weinmüllner

 

Hands-on trainings (HOT)

  • 2D culture of renal cells: primary RPTECs, RPTEC/TERT1 cells, urine-derived kidney cells (thawing, passaging, seeding into plates, chemical treatment, TEER measurement): Paul Jennings, Eva-Anne Subileau, Matthias Wieser
  • 3D culture of renal cells: spheroid culture of primary RPTECs (Eva-Anne Subileau) and seeding of kidney tubules in an OrganoPlate® (Henriëtte Lanz)
  • Toxicity test: resazurin assay, analysis if lactate production; evaluation of cellular morphology, raman microspectroscopy, data analysis and interpretation (Paul Jennings, Regina Weinmüllner)

  Provided by: Medical University of Innsbruck, Environmental Agency Austria (Umweltbundesamt), Evercyte, Biopredic, Mimetas, BOKU-VIBT

Learning Outcomes

The 2 days training will give a comprehensive overview of the most relevant and robust 2D and 3D kidney cell culture models and in vitro tests applications for the assessment of kidney-associated cytotoxicity.

Course Provider Details

Course contact information

Dr Francois Busquet
CAAT Academy coordinator
caat-academy@uni-konstanz.de
University accredited:
Last update: 25/08/2016
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