This 2-days training will give a comprehensive overview of the most relevant and robust 2D and 3D kidney cell culture models and in vitro applications for the assessment of kidney-associated cytotoxicity. Topics that will be covered during the course include:

1) Regulatory aspects

2) In vitro models and techniques that are valuable to measure toxicity:

• Normal, hTERT immortalized and iPSC-derived kidney cells

• 2D and 3D kidney culture (high density transwell culture, spheroids, microfluidic chips)

• Toxicity assessment using e.g. multi-well plates and Raman microspectroscopy with deep insight into read-out and interpretation of data